Clean room construction and management approach requires the use of
2016-09-05 16:13:12
Clean room construction and management approach requires the use of
1. Studios should be short, flat area just 4 meters 2, 2.2-2.3 meters high, the interior should be smooth, smooth, no uneven edges or the like, the walls and roof of the application of impermeable material, easy to scrub and sterilization.
2. Indoor lighting area, should be able to see from the outside the situation room.
3. To ensure that sterile room clean, sterile room around the need to set up a buffer corridor, next to the corridor between the buffer and then set up, the area may be less than the sterile room.
4. Sterile rooms, corridors and buffer between the buffer and are equipped with fluorescent lamps for air disinfection with ultraviolet light, ultraviolet germicidal lamp 1 meter away from the table is appropriate, the power switch should be located outdoors.
5. Aseptic chamber should be set up buffer Importers sliding door, the door is flush with the window, the door to be closed tight, the two should be staggered, so as not to pollute the air convection.
6. Use and management of the sterile chamber
(1) sterile room should be kept clean and tidy, indoor storage is only necessary to test appliances such as alcohol lamp, alcohol, cotton, matches, tweezers, needle vaccination, ring vaccination, glass pencil. & Do not put items unrelated to the detection
(2) testing appliances, indoor tables and stools, such as maintaining a fixed position and should not be moved.
(3) every 2-3 weeks with a 2% aqueous solution of carbolic acid to clean tables, doors, windows, tables, chairs and the floor, and then disinfect the air with 3% aqueous solution of carbolic acid spray, and finally ultraviolet light disinfection and a half hours
(4) regular inspection of indoor air sterile conditions, the number of bacteria should be controlled at 10 or less, and found not to meet the requirements, should be immediately and thoroughly sterilized. Determination / sterility of sterile room: Take ordinary bouillon agar plates, each modified Martin medium plates 3 (each 9 cm diameter plates), the sterile room is set on the working positions, openings exposed half an hour, then Invert the cultured, the total number of bacteria should be measured at 37 ℃ incubator for 48 hours; the measured mold counts should be set to 27 ℃ incubator for 5 days. The total number of bacteria fungi `shall be over 10 to.
(5) before the sterile room disinfection, all items should be placed in parts of the operation of the (analyte exception), then turn on the UV light disinfection 30 minutes, time is up, turn off the UV lamp stand
(6) before entering the sterile rooms, sterile clothes must be replaced in the buffer room, work shoes and work hats.
(7) The operation should be carried out strictly in accordance with the provisions of aseptic operation, operation less talk, not noisy, to remain sterile environment.
Clean room construction and management approach requires the use of
Microbiological testing with equipment and premises must be sterilized or disinfected, different objects using different processing methods.
1. Autoclave: overalls, masks, diluent, etc., set high pressure sterilization pot, generally use 121 ℃ sterilization for half an hour, of course, different media have different requirements, should be dealt with separately
2. Flame sterilization: inoculating needle, ring vaccination direct flame sterilization
3. Hot, dry sterilization: a variety of glassware, syringes, straws, dry box placed in 160 ℃ sterilization 2 hours
4. General disinfection: a sterile room bench, table, test tube rack, scales, container or packaging to be tested were unable to be sterilized must be disinfected using other methods, the use of a 2% aqueous solution of carbolic acid or Lysol children wipe disinfection work hand personnel also use this method to disinfect
5. Air disinfection: ultraviolet light to open 30-60 minutes.
Clean room construction and management approach requires the use of
1. First disinfecting the sterile room space, opening an ultraviolet lamp for 30-60 minutes
2. Testing and related equipment, front moved into the sterile room must be sterilized separately
3. The operator must be hand cleaned and disinfected, sterile Wear work clothes, hats and shoes, to enter the sterile room
4. Into a sterile chamber again after hand disinfection before inspection operation
Clean room construction and management approach requires the use of
1. Action should be light, not too fast, so as not to stir the air pollution increased; glassware should handle it gently, so as not to pollute the environment damage
2. Operation should be carried out in the area near the flame.
3. Ring vaccination, vaccination needles and other metal equipment before and after use is required burning, burning the first time through the inner flame that burned residue after drying sterilization
4. When using the pipette, do not direct suction mouth, blowing straw, but the ball must wash the ear operation.
5. Good openings when viewed flat, dampened For colony inspection, must operate close to the flame zone, the cover plate can not be wide open, but the upper and lower lid appropriately slotted.
6. When bacteria smear suspicious, you should use the clip holding the slide, do not take a direct hand slide to prevent pollution, used slides should be placed in disinfectant immersion disinfection, and then washed.
7. End of the work, packed bench samples and equipment, and finally wipe the table with disinfectant.
Sterile room regulations
1. sterile room floors, countertops, other surfaces daily morning wiping 1 times. Clean Bench daily morning wipe clean with 75% alcohol disinfection station before each use ready goods, the Clean Bench UV sterilized before use after 30 minutes.
2. From the outside into the interior of the sterile surface of the article all use 75% alcohol or ultraviolet irradiation pre-sterilized. 75% alcohol before aseptic sterilization palms, back of the hand. Strict aseptic whole operation.
3. Each person use, remove all items in the table with disinfectant spray disinfection article homing after use, garbage out of the sterile room. Continuous use of time more than one hour, please make an appointment for registration.
4. sterile room dedicated computer bioreactor, with No Admittance. Use the dust cover after cover, to prevent direct UV irradiation.
5. aseptic chamber regularly sterilization: 3-5% carbolic acid solution and sprayed with 2-3% Lysol wipe the desktop, stools, sterile room disinfection spray, UV sterilization for 30 minutes. once a week.
6. Availability of sterile aseptic chamber regular inspection: sterilized after inoculation indoor table and desk and put on a medium plate and exposed for 15 minutes, covered, 37 ℃ for 24 hours, colonies per plate of ≤4 is considered good effect, extend the exposure time or otherwise need to strengthen other measures. once a week.
7. sterilized items in the specified location, indicating the date of sterilization, autoclaving items can be stored for 7-14 days, expired can not be reused, need to re-packaging sterilization.
8. All ready to enter the sterile room working staff must first understand the basic requirements of the cell culture and practices, and the necessary training.
Related experiments required to submit plans before entering a sterile chamber 9.: including the use of cell type, quantity; required reagent type, reagent is toxic; broth species; test time. Not welcome no plan and is not feasible to test a person aimlessly.
Training 10. Each cell researchers; location within the incubator; broth; digestive; disinfectant items; sterilize items placed position so immobilized, and well marked. Prohibit indiscriminate use cross, misplacing, causing possible contamination expanded, all the researchers may not be used without their permission items and location of others.
11. The versatility of liquid (such as broth, digestive, physiological saline, calcium solution) formulated by the unified management, personnel management, quality control and facilitate accounting researchers. Researchers needed to apply, and well marked, so that someone special was dedicated strictly prohibited without their permission mess with others items. Only in broth after use can then apply for a new bottle and prevent waste.
12. Graduate Experiment with your saved items receive a registration management. Acid bulb before the experiment, packaging and sterilization, after the experiment promptly wash, dry and homing.
1. Studios should be short, flat area just 4 meters 2, 2.2-2.3 meters high, the interior should be smooth, smooth, no uneven edges or the like, the walls and roof of the application of impermeable material, easy to scrub and sterilization.
2. Indoor lighting area, should be able to see from the outside the situation room.
3. To ensure that sterile room clean, sterile room around the need to set up a buffer corridor, next to the corridor between the buffer and then set up, the area may be less than the sterile room.
4. Sterile rooms, corridors and buffer between the buffer and are equipped with fluorescent lamps for air disinfection with ultraviolet light, ultraviolet germicidal lamp 1 meter away from the table is appropriate, the power switch should be located outdoors.
5. Aseptic chamber should be set up buffer Importers sliding door, the door is flush with the window, the door to be closed tight, the two should be staggered, so as not to pollute the air convection.
6. Use and management of the sterile chamber
(1) sterile room should be kept clean and tidy, indoor storage is only necessary to test appliances such as alcohol lamp, alcohol, cotton, matches, tweezers, needle vaccination, ring vaccination, glass pencil. & Do not put items unrelated to the detection
(2) testing appliances, indoor tables and stools, such as maintaining a fixed position and should not be moved.
(3) every 2-3 weeks with a 2% aqueous solution of carbolic acid to clean tables, doors, windows, tables, chairs and the floor, and then disinfect the air with 3% aqueous solution of carbolic acid spray, and finally ultraviolet light disinfection and a half hours
(4) regular inspection of indoor air sterile conditions, the number of bacteria should be controlled at 10 or less, and found not to meet the requirements, should be immediately and thoroughly sterilized. Determination / sterility of sterile room: Take ordinary bouillon agar plates, each modified Martin medium plates 3 (each 9 cm diameter plates), the sterile room is set on the working positions, openings exposed half an hour, then Invert the cultured, the total number of bacteria should be measured at 37 ℃ incubator for 48 hours; the measured mold counts should be set to 27 ℃ incubator for 5 days. The total number of bacteria fungi `shall be over 10 to.
(5) before the sterile room disinfection, all items should be placed in parts of the operation of the (analyte exception), then turn on the UV light disinfection 30 minutes, time is up, turn off the UV lamp stand
(6) before entering the sterile rooms, sterile clothes must be replaced in the buffer room, work shoes and work hats.
(7) The operation should be carried out strictly in accordance with the provisions of aseptic operation, operation less talk, not noisy, to remain sterile environment.
Clean room construction and management approach requires the use of
Microbiological testing with equipment and premises must be sterilized or disinfected, different objects using different processing methods.
1. Autoclave: overalls, masks, diluent, etc., set high pressure sterilization pot, generally use 121 ℃ sterilization for half an hour, of course, different media have different requirements, should be dealt with separately
2. Flame sterilization: inoculating needle, ring vaccination direct flame sterilization
3. Hot, dry sterilization: a variety of glassware, syringes, straws, dry box placed in 160 ℃ sterilization 2 hours
4. General disinfection: a sterile room bench, table, test tube rack, scales, container or packaging to be tested were unable to be sterilized must be disinfected using other methods, the use of a 2% aqueous solution of carbolic acid or Lysol children wipe disinfection work hand personnel also use this method to disinfect
5. Air disinfection: ultraviolet light to open 30-60 minutes.
Clean room construction and management approach requires the use of
1. First disinfecting the sterile room space, opening an ultraviolet lamp for 30-60 minutes
2. Testing and related equipment, front moved into the sterile room must be sterilized separately
3. The operator must be hand cleaned and disinfected, sterile Wear work clothes, hats and shoes, to enter the sterile room
4. Into a sterile chamber again after hand disinfection before inspection operation
Clean room construction and management approach requires the use of
1. Action should be light, not too fast, so as not to stir the air pollution increased; glassware should handle it gently, so as not to pollute the environment damage
2. Operation should be carried out in the area near the flame.
3. Ring vaccination, vaccination needles and other metal equipment before and after use is required burning, burning the first time through the inner flame that burned residue after drying sterilization
4. When using the pipette, do not direct suction mouth, blowing straw, but the ball must wash the ear operation.
5. Good openings when viewed flat, dampened For colony inspection, must operate close to the flame zone, the cover plate can not be wide open, but the upper and lower lid appropriately slotted.
6. When bacteria smear suspicious, you should use the clip holding the slide, do not take a direct hand slide to prevent pollution, used slides should be placed in disinfectant immersion disinfection, and then washed.
7. End of the work, packed bench samples and equipment, and finally wipe the table with disinfectant.
Sterile room regulations
1. sterile room floors, countertops, other surfaces daily morning wiping 1 times. Clean Bench daily morning wipe clean with 75% alcohol disinfection station before each use ready goods, the Clean Bench UV sterilized before use after 30 minutes.
2. From the outside into the interior of the sterile surface of the article all use 75% alcohol or ultraviolet irradiation pre-sterilized. 75% alcohol before aseptic sterilization palms, back of the hand. Strict aseptic whole operation.
3. Each person use, remove all items in the table with disinfectant spray disinfection article homing after use, garbage out of the sterile room. Continuous use of time more than one hour, please make an appointment for registration.
4. sterile room dedicated computer bioreactor, with No Admittance. Use the dust cover after cover, to prevent direct UV irradiation.
5. aseptic chamber regularly sterilization: 3-5% carbolic acid solution and sprayed with 2-3% Lysol wipe the desktop, stools, sterile room disinfection spray, UV sterilization for 30 minutes. once a week.
6. Availability of sterile aseptic chamber regular inspection: sterilized after inoculation indoor table and desk and put on a medium plate and exposed for 15 minutes, covered, 37 ℃ for 24 hours, colonies per plate of ≤4 is considered good effect, extend the exposure time or otherwise need to strengthen other measures. once a week.
7. sterilized items in the specified location, indicating the date of sterilization, autoclaving items can be stored for 7-14 days, expired can not be reused, need to re-packaging sterilization.
8. All ready to enter the sterile room working staff must first understand the basic requirements of the cell culture and practices, and the necessary training.
Related experiments required to submit plans before entering a sterile chamber 9.: including the use of cell type, quantity; required reagent type, reagent is toxic; broth species; test time. Not welcome no plan and is not feasible to test a person aimlessly.
Training 10. Each cell researchers; location within the incubator; broth; digestive; disinfectant items; sterilize items placed position so immobilized, and well marked. Prohibit indiscriminate use cross, misplacing, causing possible contamination expanded, all the researchers may not be used without their permission items and location of others.
11. The versatility of liquid (such as broth, digestive, physiological saline, calcium solution) formulated by the unified management, personnel management, quality control and facilitate accounting researchers. Researchers needed to apply, and well marked, so that someone special was dedicated strictly prohibited without their permission mess with others items. Only in broth after use can then apply for a new bottle and prevent waste.
12. Graduate Experiment with your saved items receive a registration management. Acid bulb before the experiment, packaging and sterilization, after the experiment promptly wash, dry and homing.
